Researchers used gene knockdown and overexpression in kidney cells, along with biochemical assays to measure mitochondrial activity, NAD⁺ levels, and protein interactions related to PPDPF function.

a technique known as gene knockdown. 8 By delivering dsRNA that are complementary to a target gene’s mRNA, researchers redirect the RNAi pathway from its canonical functions to cleave the target and ...

The method, reported today (October 5) in Nature Materials, provides a new way to study the functions of adipocytes—a notoriously intractable cell type—and suggests an avenue for gene therapy to ...

Transform the verified clones into the cell lines of interest to test their ability to knock down gene expression. The vector(s) may be transiently transfected into any commonly used cell line ...

They have been successfully used for small interfering RNA (siRNA) delivery, achieving high gene knockdown efficiency in various plant species. 3,4 Carbon nanotubes (CNTs)—both single-walled and multi ...

Shihabeddin et al utilized single-cell RNA-Seq analysis of adult P23H zebrafish animals to identify transcription factors (e2fs, Prdm1a, Sp1) expressed selectively in neural progenitors and immature ...