60µg of sheared chromatin were used for each ChIP and were incubated ON with the following antibodies: 7.5 µg α-H2A.Z (Abcam, ab4174), 7.5 µg α-H2A (Cell Signaling, D603A) and 7.5 µg α-HA ...

The repressor-element 1-silencing transcription/neuron-restrictive silencer factor (REST/NRSF) controls hundreds of neuron-specific genes. We showed that REST/NRSF ...

In the CNS, calpains exist in two major isoforms: µ-calpain (or calpain-I) and m-calpain (or calpain-II). To determine whether these calpains showed changes in their expression after SCI, we performed ...

1 mM EDTA (pH 8.0), 150 mM NaCl, 1 mM NaF, 1 mM PMSF, 1 mM Na3VO4, and a 1:100 dilution of protease inhibitor mixture (Sigma-Aldrich), After incubation on ice for 1 h, the lysates were centrifuged at ...