Specially the authors evaluated the effect of ITCs (AITC, BITC, PITC, SFN and IPRITC) on the “alarmone” penta/tetraphosphate (p)ppGpp that was able to influence the activity of RNA polymerase and the development of bacteriophage and Shiga toxin production.

The reaction of isothiocyanate with thiols is a reversible reaction and the equilibrium shown in Fig. 6.2 is maintained. They also react with amino acids and amine groups to form other products (see Scheme 6.1).

Sep 1, 2011 · Isothiocyanates may mediate their effects via direct protein modification or indirectly by disruption of redox homeostasis and increased thiol oxidation. Some target proteins have been identified, but in-depth searches with new techniques are needed to reveal novel targets.

Dec 20, 2023 · Pahar et al. recapitulate the explored up-to-date reactions of isocyanates and isothiocyanates with group 13 elements, including discussion of C=N vs. C=O(S) bond activation, [2+2] cycloaddition reactions, and FLP-assisted stoichiometric reactions.

The reaction of isothiocyanates with compounds carrying an active hydrogen give adducts which undergo cyclization spontaneously or by addition of external reagents to yield heterocycles.

Dec 9, 2011 · Results: Thiolated isothiocyanate showed electrophilic response, targeted cellular Hsp90β, and stimulated heat shock response. Conclusion: Thiolated isothiocyanates are an active metabolite that could contribute to cellular responses through transthiocarbamoylation of …

Nov 4, 2015 · The RNA extraction methods using phenol and guanidine isothiocyanate solution and a silica membrane column can be useful for obtaining high integrity RNA from cell/agarose constructs rich in polysaccharide and extracellular matrix.

Isothiocyanates are the more common isomers of thiocyanates, which have the formula R−S−C≡N. Many isothiocyanates from plants are produced by enzymatic conversion of metabolites called glucosinolates. A prominent natural isothiocyanate is allyl isothiocyanate, also known as mustard oils.

In this work, an investigation of a multicomponent reaction (MCR) using isocyanides, elemental sulfur and amines revealed that isocyanides can be converted to isothiocyanates using sulfur and catalytic amounts of amine bases, especially DBU (down to 2 mol%).

This molecule was evaluated against fluorescein isothiocyanate, a prevalent labelling agent. The reactivity and selectivity of phenyl- and benzyl isothiocyanate were compared at different pHs, and their activity was tested on several protein targets.